Gliclazide Does Not Fully Prevent 2-Deoxy-D-Ribose-Induced Oxidative Damage Because It Does Not Restore Glutathione Content in a Pancreatic β-Cell Line
Figure 2
Effects of gliclazide and NAC on dRib-induced apoptosis of HIT-T15 cells. Cells were preincubated with gliclazide or NAC for 30 min and then cultured with 50 mM dRib for 24 h. Cells were stained with annexin V-FITC (horizontal axis) and PI (longitudinal axis) and analyzed using flow cytometry. The graph is representative of four independent experiments. (a) Vehicle (0.04% DMSO); (b) 50 mM dRib; (c) 10 μM gliclazide + 50 mM dRib; (d) 50 μM gliclazide + 50 mM dRib; (e) 100 μM gliclazide + 50 mM dRib; (f) 1 mM NAC + 50 mM dRib.
