Gliclazide Does Not Fully Prevent 2-Deoxy-D-Ribose-Induced Oxidative Damage Because It Does Not Restore Glutathione Content in a Pancreatic β-Cell Line
Figure 4
Scavenging effects of gliclazide and NAC on hydroxyl radical generation. (a) Representative ESR spectra of DMPO-hydroxyl radical conjugates measured in the Fenton reaction. (b) Comparison of hydroxyl radical-scavenging activity between gliclazide and NAC in the reaction as in (a). Reaction mixtures contained 60 mM DMPO, 2 mM FeSO4, and 2 mM H2O2 in DPBS with and without various concentrations of gliclazide or NAC dissolved in 0.4% ethanol. The results are the mean ± SD from four independent experiments. ** versus vehicle- (0.4% ethanol)-treated control evaluated by one-way ANOVA with Duncan’s post hoc test. NS: no significant difference from the 1 mM NAC group.
