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Oxidative Medicine and Cellular Longevity
Volume 2012, Article ID 390678, 7 pages
Research Article

Gliclazide Does Not Fully Prevent 2-Deoxy-D-Ribose-Induced Oxidative Damage Because It Does Not Restore Glutathione Content in a Pancreatic β-Cell Line

Department of Internal Medicine, Jeju National University School of Medicine, 66 Jejudaehakno, Jeju 690-756, Republic of Korea

Received 30 August 2011; Revised 14 October 2011; Accepted 20 October 2011

Academic Editor: Neelam Khaper

Copyright © 2012 Gwanpyo Koh et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

“dRib produced the intracellular glutathione depletion in a dose–dependent manner” We used two methods based on a different principle, mBCl assay and a Cayman glutathione kit, for assessing the changes in intracellular GSH levels. They showed 6h–stimulation with various concentrations of dRib significantly and dose–dependently decreased the intracellular amounts of reduced and total glutathiones in HIT–T15 cells. These results clearly demonstrated that dRib depleted the intracellular GSH content in HIT–T15 cells.

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