Gliclazide Does Not Fully Prevent 2-Deoxy-D-Ribose-Induced Oxidative Damage Because It Does Not Restore Glutathione Content in a Pancreatic β-Cell Line

Table 2

Relative intracellular ROS levels in vehicle- and dRib-treated HIT-T15 cells, with or without gliclazide or NAC.

	Relative fluorescence; (fold change from control)
Control	1.0
50 mM dRib alone	97.2±15.7 **
10 μM gliclazide + 50 mM dRib	84.0±22.7
50 μM gliclazide + 50 mM dRib	63.7±17.5^††
100 μM gliclazide + 50 mM dRib	50.3±9.0^††
1 mM NAC + 50 mM dRib	1.7±0.3^††

Data are expressed as the mean ± SD of four independent experiments. **𝑃<0.01 versus control (0.04% DMSO) and ^††𝑃<0.01 versus 50 mM dRib alone evaluated by one-way ANOVA with Duncan’s post hoc test.