Metformin Rescues the Myocardium from Doxorubicin-Induced Energy Starvation and Mitochondrial Damage in Rats

Figure 5

MET reverses DOX-mediated changes in the levels of cardiac hypertrophic genes. Rats (𝑛=10/treatment type) received normal saline, DOX in a total cumulative dose of 18 mg/kg, MET (50 or 500 mg/kg, p.o., daily), or DOX plus either dose of MET. 24 h after receiving the last dose of DOX, total RNA was isolated from rat cardiac tissue using TRIzol methods and quantified spectrophotometrically at 260 nm. The mRNA levels of α-MHC (a) and β-MHC genes (b) were quantified using RT-PCR and normalized to β-actin housekeeping gene. Values represent mean of fold change ± SEM (𝑛=10). ^∗𝑃<0.05 compared with control rats. ⁺𝑃<0.05 compared with DOX-treated rats.

(a)

(b)