NF-B and Matrix-Dependent Regulation of Osteopontin Promoter Activity in Allylamine-Activated Vascular Smooth Muscle Cells
Figure 1
Stability of the allylamine-activated vascular smooth muscle cell phenotype during serial propagation in vitro. Vascular smooth muscle cells were isolated from control and allylamine-treated rats and established in primary culture or passaged for up to 25 passages using established procedures. Cells were seeded at a density of 150 cells/mm2 and incubated with 10% fetal bovine serum for four days in culture. The results shown represent the average number of cells in 5–7 replicate dishes during primary culture or at passages 3 and 25 for the two cell types. Similar patterns were observed in 3 separate experiments. The results show that allylamine cells exhibited a proliferative advantage that was sustained for multiple passages in vitro.
