NF-𝜿B and Matrix-Dependent Regulation of Osteopontin Promoter Activity in Allylamine-Activated Vascular Smooth Muscle Cells

Table 2

Site-directed mutagenesis of opn promoter constructs.

Binding site		Sequence
VDRE	Original	5′ CCTGGAAGGGTCATATGGTTCAGCTCCGAGG 3′
	Mutated	5′ CCTGGAAtcGTtcTATGGaTCtGCTCCGAGG 3′
AP-1	Original	5′ TCGTGTTGAGTCATTCCTGTGGGC 3′
	Mutated	5′ TCGTGTTacaTCATTCCTGTGGGC 3′
NF-κB	Original	5′ AGGATTTGTGGAATTTCCCTGCACAGC 3′
	Mutated	5′ AGGATTTGaaGAATaaCaCTGCACAGC 3′
USF1	Original	5′ AAACAAACTCATGTGGGTGTCGTGCTT 3′
	Mutated	5′ AAACAAACTCAaaTGGGTGTCGTGCTT 3′

Transcription factor binding sites were mutated using Stratagene’s QuikChange and Site-Directed Mutagenesis Kit. Binding sites are underlined, and mutated bases shown in lowercase.