Detailed Analysis of Apoptosis and Delayed Luminescence of Human Leukemia Jurkat T Cells after Proton Irradiation and Treatments with Oxidant Agents and Flavonoids

Figure 1

Apoptosis and cell-cycle distributions assessed at 24 and 48 h after treatment of Jurkat cells with the vehicle (Ctrl), with 0.5, 5 or 50 μM QC for 24 h (Q0.5, Q5, Q50), 10 or 50 μM QC for 1 h (Q10*, Q50*), 0.5 μM EGCG for 24 h (E0.5), 250 μM MD for 20 min. (M250), 100, or 500 μM H₂O₂ for 20 min. (H100 or H500) or after combined treatments (QC or EGCG preincubation followed by addition of 250 μM MD or 100/500 μM H₂O₂ for 20 min.) and after irradiation with 2 Gy or 10 Gy of protons (IR 2 Gy, IR 10 Gy). Apoptotic rates (a), G₀/G₁ (b), S-phase (c), and G₂/M (d) cell fractions are indicated.

(a)

(b)

(c)

(d)