Detailed Analysis of Apoptosis and Delayed Luminescence of Human Leukemia Jurkat T Cells after Proton Irradiation and Treatments with Oxidant Agents and Flavonoids
Figure 3
Quercetin decreases clonogenic survival and the cellular content of NAD(P)H in Jurkat cells. (a) Dose response of clonogenicity (S) was fitted to an exponential function (curve) of the form S(%) = 100 × exp(−D/), where represents the dose of QC applied for 1 h and the characteristic dose derived from the fit was = 158.5 μM. Data are expressed as mean ± standard deviation of 4–6 separate determinations. (b) The ratio between NAD(P)H fluorescence of treated versus control cells (relative NAD(P)H) obtained in steady state after addition of QC to cell suspensions decreases with the level of QC. (c) Representative recordings of NAD(P)H fluorescence relative to the resting value in cell suspensions before and after addition of QC at various levels indicated near each trace.
