Isoliquiritigenin-Induced Differentiation in Mouse Melanoma B16F0 Cell Line
(a) Effects of ISL on proliferation of B16F0 cells. Cell inhibition rate was determined by MTT assay. Treatment of B16F0 cells by ISL (0, 5, 10, 15, 20, and 25 μg/mL) for 24 h or 48 h was resulted in a significant concentration and time-dependent inhibition on cell viabilities. The data represent the means ± SD from nine samples of three independent experiments. (b) Phase-contrast micrographs (400x) show the morphology changes of B16F0 cells treated by ISL (0, 5, 10, and 15 μg/mL) for 48 h. (c) The extracellular melanin contents in ISL-treated B16F0 cells for 48 h. versus control (0 μg/mL). (d) The intracellular melanin contents in ISL-treated B16F0 cells for 48 h. , versus control.