Induction of Mitochondrial Changes Associated with Oxidative Stress on Very Long Chain Fatty Acids (C22:0, C24:0, or C26:0)-Treated Human Neuronal Cells (SK-NB-E)
Figure 2
Evaluation of the effects of C22:0, C24:0, and C26:0 on SK-NB-E cells in terms of cell growth and cell morphology. SK-NB-E cells were cultured for 48 h in the absence (control) or presence of α-cyclodextrin (1 mg/mL) (vehicle), or a VLCFA (C22:0, C24:0, or C26:0) used at 0.1–20 μM. (a) Number of viable cells per well of 12 well plates determined in the presence of trypan blue; (b) observation with phase contrast microscopy. The arrows point towards aggregates of round cells. Data shown are mean ± SD from at least three independent experiments conducted in duplicates. Significance of the difference between vehicle- and fatty acid-treated cells is indicated by *(Mann-Whitney test; *). No significant difference was observed between control and vehicle-treated cells.
