Induction of Mitochondrial Changes Associated with Oxidative Stress on Very Long Chain Fatty Acids (C22:0, C24:0, or C26:0)-Treated Human Neuronal Cells (SK-NB-E)
Figure 4
Flow cytometric evaluation of the effects of C22:0, C24:0, and C26:0 on mitochondrial transmembrane potential. SK-NB-E cells were cultured for 48 h in the absence (control) or presence of α-cyclodextrin (1 mg/mL) (vehicle), or a VLCFA (C22:0, C24:0, or C26:0) used at 0.1–20 μM. The mitochondrial transmembrane potential was determined after staining with DiOC6(3) in order to quantify the cells with depolarized mitochondria. Data shown are mean ± SD from three independent experiments conducted in duplicate. Significance of the difference between vehicle- and fatty acid-treated cells is indicated by *(Mann-Whitney test; *). No significant difference was observed between control and vehicle-treated cells.
