Induction of Mitochondrial Changes Associated with Oxidative Stress on Very Long Chain Fatty Acids (C22:0, C24:0, or C26:0)-Treated Human Neuronal Cells (SK-NB-E)
Figure 7
Analysis by fluorescence microscopy and flow cytometry of the effects of C22:0, C24:0, and C26:0 on mitochondrial topography and mitochondrial mass. SK-NB-E cells were cultured for 48 h in the absence (control) or presence of α-cyclodextrin (1 mg/mL) (vehicle), or a VLCFA (C22:0, C24:0, or C26:0) used at 10 μM. Mitochondria were identified by staining with MitoTracker Red. (a) Evaluation of mitochondrial topography by fluorescence microscopy; whereas some fluorescent dots are often observed in the cytoplasm of untreated (control) and α-cyclodextrin (vehicle)-treated cells, a more diffuse and irregular staining pattern is detected under treatment with VLCFAs. (b) Quantification of mitochondrial mass by flow cytometry. Data shown are representative of at least two independent experiments.
