Downregulation of Oxidative and Nitrosative Apoptotic Signaling by L-Carnitine in Ifosfamide-Induced Fanconi Syndrome Rat Model
Figure 6
Effects of ifosfamide (IFO), L-carnitine and their combination on mRNA expression of Caspase9 (a), Caspase3 (b) and Bcl2 (c) in rat kidney tissues. Rats were randomly divided into 4 different groups of 10 animals each: Control, L-carnitine, IFO, and IFO-carnitine supplemented. Carnitine supplementation was induced in rats by daily intraperitoneal injection of L-carnitine (200 mg/kg/day) for 10 successive days. IFO nephrotoxicity was induced in rats by administration of IFO (50 mg/kg/day, I.P.) for 5 successive days. IFO-carnitine supplemented rats were given the same doses of L-carnitine (200 mg/kg/day) for 5 days before and 5 days concomitant with IFO (50 mg/kg/day, I.P.). At the end of the treatment protocol, expressions levels of Caspase9, Caspase3 and Bcl2 were measured in kidney tissues. Data are presented as mean ± S.E.M. (). * and # indicate significant change from control and IFO, respectively, at using ANOVA followed by Tukey-Kramer as a post-ANOVA test.
