Figure 4: Effect of pine pollen on the BSA-AGEs formation in vitro. BSA alone at 100 mg/mL (I), BSA plus D-galactose at 0.5 M (II), BSA plus D-galactose and pine pollen (1.2 mg/mL and 2.4 mg/mL, III and IV), and BSA plus D-galactose and AG at 100 mM (V) were incubated at 37°C for eight weeks under sterile conditions. Samples were dialyzed (10 kDa cut-off) against PBS and then the content of BSA-AGEs was determined by a commercial Elisa kit as described in Section 2. Data were results of three parallel experiments and were expressed as mean± SEM. Statistical significant difference: , versus I; , versus II.