The Critical Role of Redox Homeostasis in Shikonin-Induced HL-60 Cell Differentiation via Unique Modulation of the Nrf2/ARE Pathway
Figure 2
The changes of intercellular redox homeostasis in HL-60 cells prior to Shikonin-induced differentiation. (a) The fluorescence probes DCF and CMF were used to evaluate the level of the intracellular redox products ROS and GSH in HL-60 cells by microscopy (40x). The changes of intracellular ROS (b) and GSH (c) levels in HL-60 cells treated with 0, 50, 75, and 100 ng/mL Shikonin for 4 h. The ROS and GSH levels were calculated as the mean fluorescence intensity (MFI) per 1,000 nonnecrotic cells. (d) The changes of the intracellular GSH/GSSG ratios in HL-60 cells treated with 0, 50, 75, and 100 ng/mL Shikonin for 4 h.
