Inhibition of Mitochondrial Cytochrome <i>c</i> Release and Suppression of Caspases by Gamma-Tocotrienol Prevent Apoptosis and Delay Aging in Stress-Induced Premature Senescence of Skin Fibroblasts

<table>Representative PCR analysis for telomerase activity of control young, SIPS, and control senescent HDFs. Lane 1, molecular weight marker, lane 2, control young, lane 3, control young (heat treated), lane 4, SIPS, lane 5, SIPS (heat treated), lane 6, control senescent, and lane 7, control senescent (heat treated). Band at 36 bp represents the internal control for the assay (a). Telomerase activity (Total Product Generated, TPG) of control young, SIPS, and control senescent HDFs. Reduction in telomerase activity was observed in SIPS and control senescent HDFs (b). <sup>a</sup>denotes <svg height="11.375" id="M19" style="vertical-align:-0.1638pt" version="1.1" viewbox="0 0 58.712502 11.375" width="58.712502" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.017,-0,0,-.017,.062,11.112)"><use xlink:href="#x1D443"></use></g><g transform="matrix(.017,-0,0,-.017,15.582,11.112)"><use xlink:href="#x3C"></use></g><g transform="matrix(.017,-0,0,-.017,30.286,11.112)"><use xlink:href="#x30"></use></g><g transform="matrix(.017,-0,0,-.017,38.446,11.112)"><use xlink:href="#x2E"></use></g><g transform="matrix(.017,-0,0,-.017,42.321,11.112)"><use xlink:href="#x30"></use></g><g transform="matrix(.017,-0,0,-.017,50.481,11.112)"><use xlink:href="#x35"></use></g>
</svg> compared to control young HDFs. Data is presented as mean <svg height="9.5625" id="M20" style="vertical-align:-0.3003pt" version="1.1" viewbox="0 0 10.1 9.5625" width="10.1" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.017,-0,0,-.017,.062,9.125)"><use xlink:href="#xB1"></use></g>
</svg> SD, <svg height="11.225" id="M21" style="vertical-align:-0.1638pt" version="1.1" viewbox="0 0 36.237499 11.225" width="36.237499" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.017,-0,0,-.017,.062,10.963)"><use xlink:href="#x1D45B"></use></g><g transform="matrix(.017,-0,0,-.017,13.305,10.963)"><use xlink:href="#x3D"></use></g><g transform="matrix(.017,-0,0,-.017,28.008,10.963)"><use xlink:href="#x36"></use></g>
</svg>.</table>

Oxidative Medicine and Cellular Longevity

fig4

Figure 4

Figure 4: Inhibition of Mitochondrial Cytochrome <i>c</i> Release and Suppression of Caspases by Gamma-Tocotrienol Prevent Apoptosis and Delay Aging in Stress-Induced Premature Senescence of Skin Fibroblasts 

Figure 4 | Inhibition of Mitochondrial Cytochrome c Release and Suppression of Caspases by Gamma-Tocotrienol Prevent Apoptosis and Delay Aging in Stress-Induced Premature Senescence of Skin Fibroblasts 