Inhibition of Mitochondrial Cytochrome c Release and Suppression of Caspases by Gamma-Tocotrienol Prevent Apoptosis and Delay Aging in Stress-Induced Premature Senescence of Skin Fibroblasts

Figure 6

Flow cytometry analysis of active caspase-3, caspase-8, and caspase-9 with FITC/PI double staining in HDFs (a). Quantitative analysis of apoptotic cells population for active caspase-3, caspase-8, and caspase-9. (b). Data are expressed as means ± SD, 𝑛=6. ^adenotes 𝑃<0.05 compared to control young, ^b𝑃<0.05 compared to GTT, ^c𝑃<0.05 compared to SIPS, and ^d𝑃<0.05 compared to control senescent cells.

(a)

(b)