Oxidative Stress Posttranslationally Regulates the Expression of Ha-Ras and Ki-Ras in Cultured Astrocytes
Figure 3
Posttranslational regulation of Ha-Ras and Ki-Ras by hydrogen peroxide in cultured astrocytes. RT-PCR analysis of Ha-Ras and Ki-Ras mRNA in control astrocytes (−) and in astrocytes treated with hydrogen peroxide for 5 min (+) is shown in (a). Immunoblot analysis of Ha-Ras and Ki-Ras in pan-Ras immunoprecipitates from cultures pretreated for 15 min with cycloheximide (CHX, 10 μg/mL) and then exposed to hydrogen peroxide for 5 min is shown in (b). Note that CHX did not affect the rapid increase in Ha-Ras and Ki-Ras levels induced by hydrogen peroxide. The immunoblot has been repeated twice with identical results.
