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Oxidative Medicine and Cellular Longevity
Volume 2012, Article ID 819310, 13 pages
http://dx.doi.org/10.1155/2012/819310
Clinical Study

Characterization of Blood Oxidative Stress in Type 2 Diabetes Mellitus Patients: Increase in Lipid Peroxidation and SOD Activity

1Rede Nordeste de Biotecnologia (RENORBIO), Instituto de Química e Biotecnologia, Universidade Federal de Alagoas (UFAL), Campus A. C. Simões, Avenida Lourival Melo Mota, s/n, Tabuleiro dos Martins, 57072-970 Maceió, AL, Brazil
2Faculdade de Nutrição/Universidade Federal de Alagoas (FANUT/UFAL), Campus A. C. Simões, Avenida Lourival Melo Mota, s/n, Tabuleiro dos Martins, 57072-970 Maceió, AL, Brazil
3Laboratório de Reatividade Cardiovascular, Instituto de Ciências Biológicas e da Saúde, Universidade Federal de Alagoas (LRC/ICBS/UFAL), Pça Afrânio Jorge, s/n, Prado, 57010-020 Maceió, AL, Brazil
4Centro de Estudos em Estresse Oxidativo, Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde/Universidade Federal do Rio Grande do Sul (CEEO/ICBS/UFRGS), Rua Ramiro Barcelos 2600 Anexo, Bairro Santana, 90035-003 Porto Alegre, RS, Brazil
5Laboratório de Nutrição em Cardiologia, Faculdade de Nutrição/Universidade Federal de Alagoas (FANUT/UFAL), Campus A. C. Simões, Avenida Lourival Melo Mota, s/n, Tabuleiro dos Martins, 57072-970 Maceió, AL, Brazil

Received 27 June 2012; Revised 14 August 2012; Accepted 15 August 2012

Academic Editor: Felipe Dal-Pizzol

Copyright © 2012 Suziy de M. Bandeira et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

This study evaluated the oxidative stress through enzymatic and nonenzymatic biomarkers in diabetic patients with and without hypertension and prediabetics. The SOD and CAT (in erythrocytes) and GPx (in plasma) enzymatic activities, plasma levels of lipid peroxidation, and total thiols were measured in the blood of 55 subjects with type 2 diabetes and 38 subjects without diabetes (9 pre-diabetics and 29 controls) aged 40–86 years. The total SOD activity and the lipid peroxidation were higher in diabetics compared to nondiabetics. In stratified groups, the total SOD activity was different for the hypertensive diabetics compared to the prediabetics and normotensive controls. Lipid peroxidation was significantly higher in both groups of diabetics (hypertensive and normotensive) compared to prediabetic groups and hypertensive and normotensive controls. There was no significant difference in the CAT and GPx activities, as well as in the concentration of total thiols in the groups studied. Present data strongly suggest the involvement of oxidative stress in the pathophysiology of diabetes, revealing that the increased lipid peroxidation has a close relationship with high glucose levels, as observed by the fasting glucose and HbA1c levels. The results evidence the correlation between lipid peroxidation and DM, irrespective of the presence of hypertension.