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Oxidative Medicine and Cellular Longevity
Volume 2012, Article ID 819623, 7 pages
Research Article

Redox Status Is Critical for Stemness in Skin Equivalents

Department of Dermatology, Bundang Seoul National University Hospital, Seoul National University College of Medicine, 166 Gumi-ro, Bundang-gu, Seongnam-si 463-707, Republic of Korea

Received 1 June 2012; Accepted 30 July 2012

Academic Editor: Jing Yi

Copyright © 2012 Hye-Ryung Choi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The skin is constantly exposed to environmental oxidative stress. Skin equivalent (SE) models are three-dimensional systems in which cell-cell or cell-matrix interactions can be investigated. In this study, the effects of vitamin C or plant extracts with high antioxidant activities were tested. There was no significant difference in the epidermal thickness, but the basal cells became cuboidal when vitamin C or plant extracts were supplemented. Furthermore, immunohistochemical staining showed linear and intense staining of α6 and β1 integrin along the basement membrane in vitamin C or plant extract treated models. The p63 and PCNA were also stained. Results showed that the number of p63 and PCNA positive cells was higher in the vitamin C or plant extract treated models than in the control SEs. Although the relationship between oxidative stress and stem cells is not known, our results suggest that redox status affects the stemness and the proliferative potential of epidermal basal cells by modulating microenvironment to epidermal basal stem cells.