Heterogeneous Responses to Antioxidants in Noradrenergic Neurons
of the Locus Coeruleus Indicate Differing Susceptibility to Free Radical Content
Figure 6
Effect of Trolox and DTT treatment in [Ca2+]c and Ψm in control and impaired conditions. (a) Graph demonstrating that both antioxidants did not impact on [Ca2+]c in control conditions ( for both). (b) Graph demonstrating that 100 μM Trolox co-treatment with 1 μM CCCP increased [Ca2+]c and 1 mM DDT did not effect the normal CCCP-induced increase in [Ca2+]c ( for Trolox for DTT cotreatments, and for CCCP by itself). (c) JC-1 fluorescence demonstrating the impact of Trolox and DTT in Ψm in control and impaired (1 μM CCCP cotreatment) conditions ( for Trolox and for DTT by themselves; for Trolox and for DTT cotreatments; for CCCP by itself). All graphs show mean ± SEM.
