The Creation of Cybrids Harboring Mitochondrial Haplogroups in the Taiwanese Population of Ethnic Chinese Background: An Extensive In Vitro Tool for the Study of Mitochondrial Genomic Variations
Figure 3
Oxygen consumption of 143B osteosarcoma, ρ 0 cell, and the cybrid harboring the mtDNA haplogroup of B5. (a) Complex I-linked respiration was performed by adding 10 mM glutamate and 10 mM malate in the presence of 0.1 mM ADP. The complex I inhibitor, rotenone, terminated the respiration. (b) Complex II-linked respiration preincubated with rotenone was performed by adding succinate in the presence of 0.1 mM ADP. The complex III inhibitor, antimycin A, terminated the respiration. (c) Quantitative data of oxygen consumption expressed in slope were shown as mean ± SEM for three independent experiments.
