Oxidative Medicine and Cellular Longevity / 2012 / Article / Fig 4

Research Article

The Creation of Cybrids Harboring Mitochondrial Haplogroups in the Taiwanese Population of Ethnic Chinese Background: An Extensive In Vitro Tool for the Study of Mitochondrial Genomic Variations

Figure 4

Oxygen consumption rate and mitochondrial membrane potential of cybrids harboring B4b, F1a, B5, D5a, D4a and N9a. (a) Complex I-linked respiration was performed by adding 10 mM glutamate and 10 mM malate in the presence of 0.1 mM ADP. Complex II-linked respiration pre-incubated with rotenone was performed by adding succinate in the presence of 0.1 mM ADP. (b) The mean TMRE fluorescence value of B4b was set to 100%. Data were expressed as mean ± SEM for four independent experiments. The statistical significance of differences between groups was determined by one-way analysis of variance followed by Bonferroni post hoc test. compared to (1) B4b, (2) F1a, (3) B5, (4) D5a, and (5) D4a.

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