Dietary Phenolic Acids Act as Effective Antioxidants in Membrane Models and in Cultured Cells, Exhibiting Proapoptotic Effects in Leukaemia Cells

Figure 8

Effect of phenolic compounds on apoptosis. HEL cells were incubated with different compounds (5, 10, 50, or 100 μM) for 20 hours, then cell lysates were subjected to SDS-PAGE and Western blotting with the indicated antibodies as described in Section 2. Tubulin detection was used as a control. Representative immunoblots are shown. (a): anti-caspase 3; (b): anti-Bax and anti-Bcl-2; (e): anti-p-Akt. (c): Densitometric analysis of three independent Western blot assays for cleaved caspase 3 (17 kDa fragment). (d): Bax/Bcl-2 ratio from densitometric analysis of three independent experiments.^∗𝑃<0.05, significantly different from control cells; ^∗∗𝑃<0.01, significantly different from control cells; ^∗∗∗𝑃<0.001, significantly different from control cells.