Figure 1: Cellular pathways and network analysis of proteins reported to be increasingly modified in senescent WI-38 human fibroblasts. (a) Primary subcellular location of modified proteins previously reported in senescent fibroblasts. Rare primary localization terms were grouped as Other representing less than 6% of total identifications. (b) Proteins were grouped by canonical pathways through the use of Ingenuity Pathways Analysis (Ingenuity Systems, http://www.ingenuity.com/). The bars represent the canonical pathways identified, named in the -axis. The -axis shows the −log of the P value calculated based on Fisher’s exact test. The dotted line represents the threshold above which there are statistically significantly more genes in a biological function than expected by chance. (c) Protein networks were obtained using Ingenuity Pathway. Proteins in red correspond to some of the proteins identified as increasingly modified in senescent fibroblasts. White open nodes indicate proteins not identified in this analysis, but associated with the regulation of some of the proteins identified. Information about the analysis of biological functions and pathways as well as network interactions is available at the Ingenuity Pathway Analysis website. A line denotes binding of proteins, whereas a line with an arrow denotes “acts on.” A dotted line denotes an indirect interaction.