Quercetin and Sesamin Protect Dopaminergic Cells from MPP⁺-Induced Neuroinflammation in a Microglial (N9)-Neuronal (PC12) Coculture System

Figure 2

IL-6 (a), IL-1β (b) and TNFα (c) protein concentration in N9 microglial cells treated first for 3 h with quercetin or sesamin and then with or without MPP⁺. After 24 h, supernatants were collected and ELISA was performed as described in Materials and Methods. MPP⁺ induced marked increases of IL-6 (a), IL-1β (b) and TNFα (c) protein concentration. When N9 microglial cells were treated with quercetin or sesamin 3 h prior to MPP⁺, we detected significant declines of IL-6, IL-1β and TNFα protein concentration (MPP⁺ Quercetin, MPP⁺ Sesamin). 𝑁=3, ***𝑃<0.001 and *𝑃<0.05 compared to the controls (Ctrl), ^◊◊𝑃<0.01 and ^◊𝑃<0.05 compared to MPP⁺, as determined by 1-way ANOVA, followed by Tukey’s multiple comparison test.

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