Quercetin and Sesamin Protect Dopaminergic Cells from MPP⁺-Induced Neuroinflammation in a Microglial (N9)-Neuronal (PC12) Coculture System

Figure 4

Effects of quercetin and sesamin on MPP⁺-induced superoxide anion (^•O₂⁻) in N9 microglia cells. (a) Fluorescence photomicrographs. Ctrl: cells were treated with control medium. quercetin, sesamin: cells were treated with quercetin or sesamin in control medium. MPP⁺: cells were treated with 500 μM MPP⁺. MPP⁺quercetin or MPP⁺ sesamin: cells were treated with quercetin or sesamin plus MPP⁺. A marked red signal was evident only in neuronal PC12 cells treated with MPP⁺. Red fluorescence was less intense in cells treated with control medium (Ctrl) or when quercetin or sesamin was added to MPP⁺ medium (MPP⁺quercetin or MPP⁺sesamin). Magnification 400x. 𝑛=3. DDC: sodium diethyldithiocarbamate. (b) Semiquantitative image analysis. ***𝑃<0.001 and **𝑃<0.01 compared to the control (CTRL), ^◊◊◊𝑃<0.001 compared to MPP⁺, as determined by 1-way ANOVA, followed by Tukey’s multiple comparison test.

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