Astragaloside IV Inhibits Oxidative Stress-Induced Mitochondrial Permeability Transition Pore Opening by Inactivating GSK-3 via Nitric Oxide in H9c2 Cardiac Cells
Figure 6
Confocal fluorescence images of DAF-FM 0 and 20 min after exposure to astragaloside IV in H9c2 cells. (a) Astragaloside IV (50 μM) significantly increased DAF fluorescence, an effect that was reversed by the NOS inhibitor L-NAME (200 μM) and the PI3 K inhibitor LY294002 (10 μM). (b) Summarized data for DAF-FM fluorescence intensity 20 min after exposure to astragaloside IV expressed as a percentage of baseline. Data are mean ± SD for 6 independent experiments performed in duplicate. * compared to control group; # compared to astragaloside IV (ANOVA followed by Tukey’s test).
