Acute Ethanol Gavage Attenuates Hemorrhage/Resuscitation-Induced Hepatic Oxidative Stress in Rats

Figure 1

Acute ethanol gavage decreases lipid peroxidation after hemorrhage and resuscitation. Rats were gavaged with saline (ctrl) or ethanol (5 g/kg, 30% EtOH) 12 h before hemorrhagic shock and resuscitation (H/R). Sham-operated animals underwent the same surgical procedures but H/R was not carried out. Immunohistochemical staining for 4-hydroxynonenal (4-HNE) reveals areas of lipid peroxidation. 4-HNE-stained, representative liver sections from sham-operated rats are given in (a) (ctrl) and (e) (EtOH). 4-HNE-stained, representative liver sections from rats at 2 h, 24 h, and 72 h after H/R are given in (c)-(d) (ctrl) and (f)–(h) (EtOH); bar is 50 μm.