Oxidative Medicine and Cellular Longevity / 2012 / Article / Fig 1

Research Article

Group VIB Phospholipase A2 Promotes Proliferation of INS-1 Insulinoma Cells and Attenuates Lipid Peroxidation and Apoptosis Induced by Inflammatory Cytokines and Oxidant Agents

Figure 1

Influence of the inflammatory cytokines interleukin-1β (IL-1β) and interferon-γ (IFN-γ) on iPLA 2γ expression by INS-1 cells. Control INS-1 cells were incubated with vehicle alone or with various concentrations of IL-1β and IFN-γ for 16 hr, and iPLA2γ mRNA levels were then determined by quantitative PCR (panel (a)) and iPLA2γ protein levels by Western blotting (panel (b)), as described in Experimental Procedures. In panel (c), control INS-1 cells were incubated with IL-1β (5 ng/mL) and IFN-γ (80 ng/mL) for various intervals (0, 8, 16, 24, and 48 hr), at the end of which iPLA2γ mRNA levels were determined by quantitative PCR. In panels (a) and (c), mean values ± SEM ( ) are displayed, and an asterisk (*) indicates a significant difference ( ) from the condition in which the concentration (panel (a)) or time (panel (b)) parameter value was zero. The immunoblot in panel (b) is representative of four experiments.
989372.fig.001a
(a) Concentration dependence of cytokine effects on iPLA2γ mRNA
989372.fig.001b
(b) Concentration dependence of inflammatory cytokine effects on iPLA2γ protien
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(c) Time course of cytokine effects on iPLA2γ mRNA

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