Oxidative Medicine and Cellular Longevity / 2012 / Article / Fig 4

Research Article

Group VIB Phospholipase A2 Promotes Proliferation of INS-1 Insulinoma Cells and Attenuates Lipid Peroxidation and Apoptosis Induced by Inflammatory Cytokines and Oxidant Agents

Figure 4

Proliferation rates of INS-1 cell lines. Control INS-1 cells (open circles) or the iPLA2γ-Knockdown cell lines KD1 (closed circles) or KD2 (closed triangles) were seeded onto the wells of microtiter plates (density 3 × 103 cells/well) and incubated for various intervals (24, 48, or 72 hr), at the end of which cell number was estimated based on DNA content by using a fluorescent indicator as described in Experimental Procedures. Mean values ± SEM are displayed ( ). An asterisk (*) denotes a significant difference ( ) from the value for the control INS-1 cell line at the indicated time point.

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