Novel Hematopoietic Target Genes in the NRF2-Mediated Transcriptional Pathway
Figure 5
Gene expression and protein analysis of ABCB6 in NRF2KD, K562, GM12878, and K562 KEAP1KD cells. (a) Significant ABCB6 decrease in NRF2-silenced A549, BEAS-2B, and HepG2 cells treated for 8 hours with 10 μM SFN. (b) ABCB6 was not induced by 24 hours of 10 μM SFN treatment in K562 cells but responded to chemical treatment in lymphoid GM12878 cells. Protein levels of ABCB6 show an increase after treatment of K562 cells with 10 μM SFN for 5 hours compared to respective controls. (c) Similar to (b), high baseline levels of ABCB6 mRNA did not change significantly in K562 KEAP1KD cells. Gene expression values were normalized to BACT or 18S. Bars represent an average of 3–6 independent experiments ± SEM; , . (d) KEAP1KD C1 and C2 total protein show increased levels of ABCB6. Protein markers confirmed an ABCB6 band at 79 kDA, and the membrane was reprobed with β-actin as a loading control.
