Study of Possible Mechanisms Involved in the Inhibitory Effects of Coumarin Derivatives on Neutrophil Activity
Effect of HHC and HHDC on PKC activity. Human isolated neutrophils (/mL) were incubated for 30 min with HHC or HHDC (1, 10, and 100 μmol/L) and stimulated with PMA (0.15 μmol/L) at 37°C for 3 min. PKC activity was measured by ELISA kit in the supernatant of cell lysate. The values were calculated as percentage of stimulated (PMA) control. Control value given as relative kinase activity (450 nm) per 1 mg of protein was 9 708 ± 1 168. Mean ± SEM, . **, * as compared with the control (PMA) in the absence of the substances tested.