Study of Possible Mechanisms Involved in the Inhibitory Effects of Coumarin Derivatives on Neutrophil Activity

Table 1

Cytotoxic effect of HHC and HHDC evaluated by means of ATP liberation.

Concentration (μmol/L)	ATP liberation (nmol/L)
	HHC	HHDC
0	13.53±1.59	12.38±1.22
1	12.63±1.67	11.99±1.24
10	10.25±0.37	12.57±1.14
100	7.91±0.41	12.94±1.01

The data express ATP liberation from 30,000 neutrophils.
0: untreated neutrophils, 1–100 μmol/L: treated by HHC or HHDC, total (159±12 nmol/L) represents ATP content as determined immediately after complete neutrophil destruction. Mean ± SEM, 𝑛=6.