Blocking Type I Interferon Signaling Rescues Lymphocytes from Oxidative Stress, Exhaustion, and Apoptosis in a Streptozotocin-Induced Mouse Model of Type I Diabetes
Figure 3
IFN-α decreases lymphocyte proliferation during diabetes. The CFSE fluorescence intensity for one representative experiment per group of mice was measured by flow cytometry, as shown in (a). The data acquired for five individual mice per group are shown in (b). To calculate the mitogen-induced specific proliferation of PBMCs, we subtracted the spontaneous proliferation (percentage of unstimulated cells in the absence of the mitogen) from the mitogen-induced proliferation (percentage of mitogen-stimulated cells). * for diabetic versus control; # for diabetic + anti-IFNAR1 versus diabetic; + for diabetic + anti-IFNAR1 versus control.
