Blocking Type I Interferon Signaling Rescues Lymphocytes from Oxidative Stress, Exhaustion, and Apoptosis in a Streptozotocin-Induced Mouse Model of Type I Diabetes

Figure 4

Increased lymphocyte apoptosis during diabetes. Isolated PBMCs were fixed and permeabilized, and the DNA was stained by incubating cells for 1 h in 40 μL/mL propidium iodide and 100 μL/mL DNase-free RNase in PBS. The samples were then analyzed by assessing the FL2 red fluorescence on a linear scale. The percentage of cells undergoing apoptosis was determined using flow cytometry. The results are expressed as the mean apoptotic cells ± SEM (𝑛=6). *𝑃<0.05 for diabetic versus control; ^#𝑃<0.05 for diabetic + anti-IFNAR1 versus diabetic; ⁺𝑃<0.05 for diabetic + anti-IFNAR1 versus control.