Quercetin Induces Mitochondrial Biogenesis through Activation of HO-1 in HepG2 Cells
Figure 5
Quercetin restores mitochondrial biogenesis in LPS-treated mice in a HO-dependent fashion. (a to d) C57BL/6 mice were injected with quercetin (50 mg/kg) for 7 alternate days, with or without SnPP (50 μmol/kg) prior to injection with quercetin, and then challenged for 24 hours with i.p. injection of LPS (10 mg/kg). (a) Expressions of PGC-1, NRF-1, and TFAM in mRNA were determined by RT-PCR. 18 S rRNA served as the standard. (b) Expression of COX IV protein was determined by Western blot analysis. α-tubulin served as the standard. (c) Expression of mtDNA content was quantified by real-time PCR. Relative amounts of mtDNA and nDNA contents were compared. (d) Expression of TNFα, IL-1β, and IL-6 mRNA was quantified by real-time PCR. 18 S rRNA served as the standard. Results are expressed as mean ± SE of three independent experiments (/group), and representative data are shown. * compared with the uninjected control group; † compared with LPS injected mice group; # compared with mice in the LPS + quercetin group.
