Resolvin D1 Reverts Lipopolysaccharide-Induced TJ Proteins Disruption and the Increase of Cellular Permeability by Regulating I κBα Signaling in Human Vascular Endothelial Cells

Figure 3

Effects of RvD1 and LPS on the ERK1/2 and IκBα signaling pathways in HUVECs. (a) Effect of RvD1 and LPS on ERK1/2 phosphorylation. (b) Effect of RvD1 and LPS on IκBα protein expression. (c) Effect of ERK inhibitor PD98059 on ERK1/2 phosphorylation in RvD1 + LPS group. (d) Effect of NF-κB inhibitor PDTC on IκBα protein expression in RvD1 + LPS group. Densitometric analysis of the protein levels of zo-1 and occludin were shown (𝑛=3). Data were expressed as mean ± s.e.m. ^∗∗𝑃<0.01, ^∗𝑃<0.05 versus LPS; ^##𝑃<0.01 versus control; ^&&𝑃<0.01, ^&𝑃<0.05 versus LPS + RvD1.

(a)

(b)

(c)

(d)