Figure 2: Immunoblot analysis of expression of NFκB and iNOS proteins in MSCs subjected to LPS challenge. (a) Immunoblotting bands of NFκB and iNOS. (b) Densitometry histograms of iNOS bands in MSCs subjected to challenge with LPS. The presented bars indicate the relative density of iNOS protein (normalized to density of actin bands). The statistical significance was determined by Student’s -test . Conditions: MSCs were incubated with 500 ng/mL LPS for 3 h. The cells were harvested at 24 h following challenge with LPS.