Research Article

Hemeoxygenase-1 Mediates an Adaptive Response to Spermidine-Induced Cell Death in Human Endothelial Cells

Figure 2

Blockage of SPD-induced HO-1 protein expression by aminoguanidine, NAC, or PI3K/Akt inhibitor. Cells were pre-treated with aminoguanidine (a), NAC (b), or LY 294002 (PI3K/Akt inhibitor) (c) 1 h prior to the treatment of SPD. After 24 h incubation, cell lysates were prepared, and 20 μg samples of proteins were subjected to Western blotting, using anti-HO-1 antibody and anti-GAPDH.
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238734.fig.002b
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238734.fig.002c
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