Research Article

Hemeoxygenase-1 Mediates an Adaptive Response to Spermidine-Induced Cell Death in Human Endothelial Cells

Figure 3

Nrf2 nuclear translocation and ARE-luciferase reporter activity induced by SPD. Cells were treated with SPD at the indicated concentration for 4 h. (a) Nuclear extracts were prepared, and 10 μg samples of proteins were subjected to Western blotting, using an anti-Nrf2 antibody and an anti-Lamin B (a marker of nuclear protein) antibody. (b) Cells were transfected with an ARE-luciferase construct. After transfection, the cells were treated with 10 μM SPD for 6 h, and the lysates were mixed with a luciferase substrate. A luminometer was used for measurement of luciferase activity. Data are the mean ± SD of quintuplicate experiments. * versus control.
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238734.fig.003b
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