Endoplasmic Reticulum Stress and Parkinson’s Disease: The Role of HRD1 in Averting Apoptosis in Neurodegenerative Disease
Figure 3
HRD1 degrades unfolded Pael-R and suppresses Pael-R-induced cell death. (a) Neuro2a cells were transiently transfected with Pael-R and HRD1, or HRD1-mutant. At 36 h after transfection, cells were pulse-labeled with -methionine/cysteine and chased for the indicated times. The levels of -labeled Pael-R are plotted relative to the amount present at time 0. (b) HEK293 cells (control) and HEK293 cells expressing HRD1 or HRD1-mutant were transiently transfected with Pael-R. The surviving cells were stained with crystal violet. The percentage of cell death was calculated as follows: 100 – ((optical density for assay/optical density for control well) × 100). The results obtained from each cell transfected with Pael-R were compared with those obtained from cells transfected with control vector. The results are expressed as the means ± S.D. of three independent experiments performed in duplicate. Statistical analysis was performed using Student’s t-test ( versus normal) [56].
