Subacute Zinc Administration and L-NAME Caused an Increase of NO, Zinc, Lipoperoxidation, and Caspase-3 during a Cerebral Hypoxia-Ischemia Process in the Rat
Figure 3
Subacute administration of zinc and L-NAME on lipoperoxidation levels during a cerebral hypoxia-ischemic process. Malonyldialdehyde (MDA) and 4-hydroxyalkanal (4-HEA) concentrations measured by using the method described elsewhere [18, 25] were used as biomarkers of lipoperoxidation. Each value represents the mean ± SE of 5 independent experiments made in triplicate. Zn96h + CCAO: preventive subacute administration of zinc (2.5 mg/kg each 24 hours for 4 days) and common carotid-artery occlusion (CCAO) for 10 min. Zn96h + L-NM + CCAO: rats treated with zinc in the presence of an inhibitor of nitric oxide synthase (L-NAME) one-hour before the CCAO. , an ANOVA test and a post-hoc Dunnet test to compare with the control group, and , unpaired Student’s t-test to compare between groups.
