Subacute Zinc Administration and L-NAME Caused an Increase of NO, Zinc, Lipoperoxidation, and Caspase-3 during a Cerebral Hypoxia-Ischemia Process in the Rat
Figure 5
Immunohistochemistry against caspase-3 and Nissl counterstaining in slides of the hippocampus and temporoparietal cortex in zinc-treated rats in the presence or absence of L-NAME. The labels at the left side of the micrographs are cerebral regions. The immunostaining against cleaved caspase-3 is shown by the dark marks (dark arrowhead), and the Nissl stain appears in blue and pale cell (clear arrowhead). Zn96h + CCAO: preventive subacute administration of zinc (2.5 mg/kg each 24 hours for 4 days) and the common carotid-artery occlusion (CCAO) for 10 min. Zn96h + L-NAME + CCAO: rats treated with zinc in presence of an inhibitor of nitric oxide synthase (L-NAME) one-hour before the CCAO. DG: dentate gyrus of hippocampus; LV: layer V of cerebral cortex.
