Exacerbated Airway Toxicity of Environmental Oxidant Ozone in Mice Deficient in Nrf2
Figure 5
Lung Nrf2 and antioxidant expression. (a) -induced changes in expression of Nrf2 mRNA (left panels) and protein (right panels) in lung homogenates from mice after exposure to air, 0.3 ppm (top), or 2 ppm (bottom). Data presented as mean ± SEM (-4/group) after normalization to air controls. * Significantly different from air control mice (). For Western blots, pan-actin was measured as a loading control. Representative band images from replicates are shown. (b) mRNA expression of antioxidants glutathione peroxidase 2 (GPx2), heme oxygenase-1 (HO-1), and NAD(P)H:quinone oxidoreductase 1 (NQO1) in lung homogenates from mice after exposure to air, 0.3 ppm (top), or 2 ppm (bottom). Data present fold differences of each gene expression relative to air after normalization to corresponding 18 s rRNA expression. Group mean ± SEM presented ( = 3-4/group). *Significantly different from genotype-matched air control (). +Significantly different from exposure-matched mice ().
