Oxidative Stress Is Related to the Deleterious Effects of Heme Oxygenase-1 in an In Vivo Neuroinflammatory Rat Model

Figure 4

Microglial activation in the cortex (bregma −3 mm) 3 days after intrastriatal injection. (a) Sagittal rat brain representation [24]. The dotted and full lines symbolize, respectively, the site of injury (bregma +0.7 mm) and the area where Ox-42 immunohistochemistry was performed (bregma −3 mm). (b) Increasing of microglia activation in the ipsi- versus the contra-lateral hemisphere for each brain. Measurements were performed in the cortex (3 areas per hemisphere) at a distance of 3.7 mm posterior from the site of injury (bregma −3 mm). *𝑃<0.05; **𝑃<0.01. (c) Ox-42 immunochemistry in control, H, QA, H-QA, DFX-QA, and DFX-H-QA brains. 1: macroscopic view (bregma −3 mm). 2: microscopic view (×20) of activated microglia in the cortex of the ipsilateral hemisphere.