Oxidative Stress Is Related to the Deleterious Effects of Heme Oxygenase-1 in an In Vivo Neuroinflammatory Rat Model

Figure 5

Immunofluorescence of NeuN and ethidium in rat striatum 1 h after QA injection. (a), (b), and (c) represent the same areas of contra- and ipsi-lateral striatum (1 and 2, resp.) of control (𝑛=5), QA (𝑛=5), H-QA (𝑛=5), ZnPP-H-QA (𝑛=5), and DFX-H-QA (𝑛=3) groups. Magnification was ×20 (×120 for inserts). Data are means±SEM. Bars: 20 μm. (a) NeuN (green channel). Neurons staining in contra- and ipsi-lateral striatum (1 and 2) for each group. Data are expressed as relative neuronal loss in the ipsilateral hemisphere versus the contralateral hemisphere (d). *𝑃<0.05 compared to respective contralateral side. (b) ROS expression analyzed with dihydroethidium (DHE) in the contra- and ipsi-lateral striatum (1 and 2) for each group. ROS measurements were performed at the same location as NeuN counting. Data are expressed as relative augmentation of ROS level in neurons in the ipsilateral hemisphere versus the contralateral hemisphere (e). *𝑃<0.05. (c) Merge representing neurons and ROS expression in contra- and ipsi-lateral striatum (1 and 2) for each group.