17-𝜷-Estradiol Counteracts the Effects of High Frequency Electromagnetic Fields on Trophoblastic Connexins and Integrins

Table 1

Primer sequences and PCR condition.

Gene	Primer sequence	𝑇𝑎; °C	Product length; (bp)	QPCR; amplification; efficiency*; (%)	No. of cycles	Reference primer; bank
Cx40	F: 5′-tcctggaggaagtacacaagc-3′; R: 5′-atcacaccggaaatcagcctg-3′	60.1	137	97.2	39	GenBank Accession; NM; 181703
Cx43	F: 5′-tcaagcctactcaactgctgg-3′; R: 5′-tgttacaacgaaaggcagactg-3′	60.4	125	98.4	39	GenBank Accession; NM; 000165
Cx45	F: 5′-atgagttggagctttctgactcg-3′; R: 5′-cggctgttctgtgttgcac-3′	60.4	174	94.5	39	GenBank Accession; NM; 005497
Int 𝛼1	F: 5′-tgctgctggctcctcactgttgtt-3′; R: 5′-gggcccacaagccagaaatcct-3′	60.6	354	95.8	39	GenBank Accession; NM; 181501.1
Int 𝛼5	F: 5′-gaaccagagccgcctgctgg-3′; R: 5′-gagcctccacggagagccga-3′	60.8	215	95.8	39	GenBank Accession; NM; 002205.2
Int 𝛽1	F: 5′-acgccgcgcggaaaagatgaatt-3′; R: 5′-acccacaatttggccctgcttg-3′	60.5	155	95.4	39	GenBank Accession; NM; 002211.3
RPL13A	F: 5′-cctaagatgagcgcaagttgaa-3′; R: 5′-ccacaggactagaacacctgctaa-3′	60.2	203	97.3	39	Pattyn et al. 2006 [34]
RPL11A	F: 5′-tgcgggaacttcgcatccgc-3′; R: 5′-gggtctgccctgtgagctgc-3′	60.1	108	96.5	39	GenBank Accession; NM; 000975.2
GAPDH	F: 5′-tgacgctggggctggcattg-3′; R: 5′-ggctggtggtccaggggtct-3′	60	134	94.6	39	GenBank Accession; NM; 002046.3

Data calculated by OpticonMonitor 3 Software (Bio-Rad).