Attenuation of 𝜷-Amyloid-Induced Oxidative Cell Death by Sulforaphane via Activation of NF-E2-Related Factor 2

Figure 3

Protective effect of NAC on A𝛽_25--35-induced apoptotic cell death. SH-SY5Y cells were treated with A𝛽_25--35 (15 𝜇M) with or without NAC (0.5 mM and 1 mM) for 24 h. (a) Cell viability was measured and calculated by MTT dye reduction assay. Data are shown as mean ± S.D. (𝑛=3). ^##𝑃<0.01, control versus A𝛽_25--35 and  ^∗𝑃<0.05 or  ^∗∗𝑃<0.01, A𝛽_25--35 versus A𝛽_25--35 + NAC. (b) Apoptotic cell death was examined by TUNEL staining. (A) Vehicle-treated control; (B) A𝛽_25--35 alone (15 𝜇M); (C) A𝛽_25--35 (15 𝜇M) + NAC (1 mM). (c) MMP was monitored by relative TMRE fluorescence intensity. (A) Vehicle-treated control; (B) A𝛽_25--35 alone (15 𝜇M); (C) A𝛽_25--35 (15 𝜇M) + NAC (1 mM).

(a)

(b)

(c)